Gene expression effects in Caenorhabditis elegans strains of feeding on Erwinia rhapontici or Escherichia coli

Dataset

Description

Diet can have a strong impact on development, to test this effect the strains N2, CB4856, three strains from Orsay (JU1581, JU1944, and JU1949), and three strains from Santeuil (JU1921, JU1930, and JU1932) were grown on two different food-sources. These sources were the standard lab food, Escherichia coli OP50, and the Gram-negative plant pathogen Erwinia rhapontici. The effect of these bacteria on development could also be measured by the start of reproduction, which was delayed while feeding on E. coli. To measure the transcriptional effects, age-synchronized strains were grown for 48 hours at 20 degrees Celsius. The impact of food-source on gene expression was determined by a transcriptional ruler based on the strain N2 (E-MTAB-7019).
Date made available12 Jul 2019
PublisherWageningen University & Research

Research Output

A rapid and massive gene expression shift marking adolescent transition in C. elegans

Snoek, L. B., Sterken, M. G., Volkers, R. J. M., Klatter, M., Bosman, K. J., Bevers, R. P. J., Riksen, J. A. G., Smant, G., Cossins, A. R. & Kammenga, J. E., 2014, In : Scientific Reports. 4, 3912.

Research output: Contribution to journalArticleAcademicpeer-review

Open Access
  • 18 Citations (Scopus)

    Cite this

    Sterken, M. G. (Creator) (12 Jul 2019). Gene expression effects in Caenorhabditis elegans strains of feeding on Erwinia rhapontici or Escherichia coli. Wageningen University & Research.