Experimental evolution of Staphylococcus aureus in macrophages: dissection of a conditional adaptive trait promoting intracellular survival

Staphylococcus aureus is a major pathogen associated with important diseases in humans and animals. Macrophages represent an important component of the innate immune response that play a keys role in S. aureus infection outcome. In order to investigate the adaptive evolution of S. aureus in response to macrophages, we developed an experimental infection assay. S. aureus strains representing major human epidemic clones were passaged many times in a macrophage cell line, accumulating mutations in an array of genomic loci. This BioProject contains the read data (Illumina) for all sequenced passages, and long read data (PacBio) for selected passages of interest.