Ethyl tert-butyl ether (EtBE) degradation by an algal-bacterial culture

  • J.M. van der Waals Deltares (Creator)
  • Caroline Plugge (Creator)
  • Marion Meima-Franke (Creator)
  • P. de Waard (Creator)
  • Paul L.E. Bodelier (Creator)
  • Hauke Smidt (Creator)
  • Jan Gerritse (Creator)

Dataset

Description

EtBE is a fuel oxygenate, made out of (bio)ethanol replacing MtBE. Biodegradation of EtBE can reduce the risk after accidental release in the environment. The oxygen produced by Scenedesmus and Chlorella was used by microorganisms to degrade EtBE using a P450 monooxygenase cytochrome. Metabolites formed during the micro-oxic EtBE degradation were tert-butyl alcohol (TBA), ethanol and CO2 determined using 13C nuclear magnetic resonance spectroscopy (NMR) and gas chromatography. Stable isotope probing (SIP) of the 13C and 12C enriched EtBE fractions showed no significant difference between phylotypes, including Halomonadaceae, Shewanellaceae, Rhodocyclaceae, Oxalobacteraceae, Comamonadaceae, Sphingomonadaceae, Hyphomicrobiaceae, Candidatus Moranbacteria, Omnitrophica, Anaerolineaceae, Nocardiaceae, and Blastocatellaceae. This study is the first study describing micro-oxic degradation of EtBE by an algal-bacterial culture.
Date made available23 Aug 2018
PublisherWageningen University

Research Output

Ethyl tert-butyl ether (EtBE) degradation by an algal-bacterial culture obtained from contaminated groundwater

van der Waals, M. J., Plugge, C., Meima-Franke, M., de Waard, P., Bodelier, P. L. E., Smidt, H. & Gerritse, J., 1 Jan 2019, In : Water Research. 148, p. 314-323

Research output: Contribution to journalArticleAcademicpeer-review

  • 6 Citations (Scopus)

    Cite this

    van der Waals, J. M. (Creator), Plugge, C. M. (Creator), Meima-Franke, M. (Creator), de Waard, P. (Creator), Bodelier, P. L. E. (Creator), Smidt, H. (Creator), Gerritse, J. (Creator) (23 Aug 2018). Ethyl tert-butyl ether (EtBE) degradation by an algal-bacterial culture. Wageningen University.