Research output per year
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Description
We present epiGBS2, a laboratory protocol based on epiGBS (Gurp et al., 2016) with a revised and user-friendly bioinformatics pipeline for a wide range of species with or without reference genome. Performance of several critical steps in epiGBS2 was evaluated against baseline data sets from Arabidopsis thaliana and Great tit (Parus major), which confirmed overall good performance of epiGBS2. We provide here the raw bisulfite sequencing data of the epiGBS2 run for Arabidopsis thaliana.
| Date made available | 31 Dec 2021 |
|---|---|
| Publisher | Netherlands Institute of Ecology (NIOO-KNAW) |
Keywords
- non-model species
- bisulfite sequencing
- reduced representation
- double digest
- de novo reference
- DNA methylation
- SNP calling
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epiGBS2: Improvements and evaluation of highly multiplexed, epiGBS-based reduced representation bisulfite sequencing
Gawehns, F., Postuma, M., van Antro, M., Nunn, A., Sepers, B., Fatma, S., van Gurp, T. P., Wagemaker, N. C. A. M., Mateman, A. C., Milanovic-Ivanovic, S., Groβe, I., van Oers, K., Vergeer, P. & Verhoeven, K. J. F., Jul 2022, In: Molecular Ecology Resources. 22, 5, p. 2087-2104Research output: Contribution to journal › Article › Academic › peer-review
Open Access14 Citations (Scopus) -
epiGBS2: an automated snakemake workflow for highly multiplexed reduced representation bisulfite sequencing
Postuma, M., van Gurp, T., Fatma, S. & Gawehns, F., 11 May 2020Research output: Non-textual form › Software
Open Access