Dose-dependent effects of dietary fat on development of obesity in relation to intestinal differential gene expression in C57BL/6J mice

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Description

Excessive intake of dietary fat is known to be a contributing factor in the development of obesity. In this study, we determined the dose-dependent effects of dietary fat on the development of this metabolic condition with a focus on changes in gene expression in the small intestine. C57BL/6J mice were fed diets with either 10, 20, 30 or 45 energy% (E%) derived from fat for four weeks (n=10 mice/diet). We found a significant higher weight gain in mice fed the 30E% and 45E% fat diet compared to mice on the control diet. These data indicate that the main shift towards an obese phenotype lies between a 20E% and 30E% dietary fat intake. Analysis of differential gene expression in the small intestine showed a fat-dose dependent gradient in differentially expressed genes, with the highest numbers in mice fed the 45E% fat diet. The main shift in fat-induced differential gene expression was found between the 30E% and 45E% fat diet. Furthermore, approximately 70% of the differentially expressed genes were regulated in a fat-dose dependent manner. Many of these genes were involved in lipid metabolism-related processes and were already differentially expressed on a 30E% fat diet. Taken together, we conclude that up to 20E% of dietary fat, the small intestine has an effective ‘buffer capacity’ for fat handling. From 30E% of dietary fat, a switch towards an obese phenotype is triggered. We further speculate that especially fat-dose dependently regulated lipid metabolism-related genes are involved in development of obesity.
Date made available26 Apr 2011
PublisherWageningen University

Research Output

Dose-dependent effects of dietary fat on development of obesity in relation to intestinal differential gene expression in C57BL/6J mice

de Wit, N. J. W., Boekschoten, M. V., Machmair, E. M., Hooiveld, G. J. E. J., de Groot, P. J., Rubio-Aliaga, I., Daniel, H. & Muller, M. R., 2011, In : PLoS ONE. 6, 4, 7 p., e19145.

Research output: Contribution to journalArticleAcademicpeer-review

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    de Wit, N. (Creator), Boekschoten, M. (Creator), Bachmair, E. (Creator), Hooiveld, G. (Creator), de Groot, P. (Creator), Rubio-Aliaga, I. (Creator), Hannelore, D. (Creator), Muller, M. (Creator) (26 Apr 2011). Dose-dependent effects of dietary fat on development of obesity in relation to intestinal differential gene expression in C57BL/6J mice. Wageningen University.