Direct target gene analyses for TCP20 using a glucocorticoid inducible TCP20 (TCP20-GR)

Dataset

Description

TCP (TEOSINTE BRANCHED1/CYCLOIDEA/PCF1) transcription factors control developmental processes in plants. We identified direct target genes of the Arabidopsis class I TCP20 protein in leaf development based on a glucocorticoid receptor induction assay and genome-wide expression studies. For this, we tagged TCP20 with a glucucorticoid receptor (GR) domain and transformed the resulting TCP20-GR construct into tcp20 knockout plants. Induction of these and wild type controls was done with Dexamethasone and Cycloheximide. Plants were harvested 8 h after induction, uninduced plants were taken as control.

Research Output

Arabidopsis Class I and Class II TCP Transcription Factors Regulate Jasmonic Acid Metabolism and Leaf Development Antagonistically.

Danisman, S., van der Wal, F., Dhondt, S., Waites, R., de Folter, S., Bimbo, A., van Dijk, A. D. J., Muino Acuna, J., Cutri, L., Dornelas, M. C., Angenent, G. C. & Immink, R. G. H., 2012, In : Plant Physiology. 159, 4, p. 1511-1523

Research output: Contribution to journalArticleAcademicpeer-review

  • 155 Citations (Scopus)

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    Danisman, S. D. (Creator), van der Wal, F. (Creator), de Folter, S. (Creator), Naouar, N. (Creator), Muino Acuna, J. M. (Creator), Angenent, G. C. (Creator), Immink, G. H. (Creator) (9 Aug 2011). Direct target gene analyses for TCP20 using a glucocorticoid inducible TCP20 (TCP20-GR). Plant Research International, part of Wageningen UR Business Unit Bioscience.