Different lipid forms of omega-3 and their effect on small intestine in mice



Omega - 3 fatty acids of marine origin exert beneficial effects on lipid metabolism and can protect against insulin resistance in high fat diet (HFD)-fed animals. Simultaneously, recent studies showed that different lipid forms could have numerous consequences regarding the regulation of energy balance, nutrient absorption, and substrate metabolism. Indeed, when omega-3 was provided as triglycerides (TG, i.e. fish oil), it induced dose-dependently the expression of genes involved in lipid metabolism as well as fatty acid oxidation in small intestine of C57BL/6 mice fed various HFDs. As the underlying mechanism(s) explaining the differences in EPA/DHA bioavailability among various lipid forms of Omega-3 is not entirely clear, we performed a mouse study (n=8 per group) using purified HFDs with control HFD based on corn oil (cHF) and part of the lipids were replaced by omega-3 fish lipids in different forms: as either TG (cHF-F), marine phospholipids (PL; Krill oil, given at two different doses Krill-low (Krill-L) and Krill-high (Krill-H)), and as wax esters in the extract from the zooplankton Calanus finmarchicus (Calanus oil CAL-L representing same omega-3 levels as Krill-L diet). As a healthy control we fed a subset of mice standard chow (STD). All mice were fed their diet for 8 weeks and after sacrifice, whole small intestine was isolated, frozen and used for RNA isolation and microarray gene expression analysis using 8x60K Agilent arrays. Results showed that PL-H versus control cHFc induced specifically metabolic lipid pathways, while TG and PL-L mainly affected cytoskeleton regulation.
Date made available13 Jul 2020
PublisherWageningen University

Research Output

Omega-3 phospholipids from krill oil enhance intestinal fatty acid oxidation more effectively than omega-3 triacylglycerols in high-fat diet-fed obese mice

Kroupova, P., van Schothorst, E. M., Keijer, J., Bunschoten, A., Vodicka, M., Irodenko, I., Oseeva, M., Zacek, P., Kopecky, J., Rossmeisl, M. & Horakova, O., 9 Jul 2020, In : Nutrients. 12, 7, 20 p., 2037.

Research output: Contribution to journalArticleAcademicpeer-review

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  • Cite this

    van Schothorst, E. (Creator), Kucharikova, P. (Creator), Bunschoten, A. (Creator), Horakova, O. (Creator), Rossmeisl, M. (Creator), Kopecky, J. (Creator), Keijer, J. (Creator) (13 Jul 2020). Different lipid forms of omega-3 and their effect on small intestine in mice. Wageningen University.