Research Output per year
Previously, we showed that dietary heme injured the colonic surface epithelium and induced hyperproliferation by changing the surface to crypt signaling. In this study we investigated whether bacteria play a role in this changed signaling. Dietary heme increased the Bacteroidetes and decreased the Firmicutes in colonic content. This shift was caused by a selective susceptibility of Gram-positive bacteria to the heme cytotoxic fecal waters, which is not observed for Gram-negative bacteria allowing expansion of the Gram-negative community. The increased amount of Gram-negative bacteria increased LPS exposure to colonocytes, however, there is no appreciable immune response detected in the heme-fed mice. There were no signs of sensing of the bacteria by the mucosa, as changes in TLR signaling were not present. This lack of microbe-host cross talk indicated that the changes in microbiota do not play a causal role in the heme-induced hyperproliferation.
|Date made available||18 Jul 2013|
Dietary heme alters microbiota and mucosa of mouse colon without functional changes in host-microbe cross-talkIJssenagger, N., Derrien, M., van Doorn, G. M., Rijnierse, A., van den Bogert, B., Muller, M. R., Dekker, J., Kleerebezem, M. & van der Meer, R., 2012, In : PLoS ONE. 7, 12, 10 p., e49868.
Research output: Contribution to journal › Article › Academic › peer-review
IJssennagger, N. (Creator), Rijnierse, A. (Creator), Muller, M. (Creator), van der Meer, R. (Creator) (18 Jul 2013). Dietary heme modulates microbiota and mucosa of mouse colon without significant host-microbe cross talk. Wageningen University.