Research output per year
Research output per year
Description
The research is about optimization and validation of capture ELISA for chicken IL-2, IL-6, IL-10, IL-12p40, and IFN-y using commercially available antibodies and recombinant cytokines.
To optimize antibody concentrations, checkerboard titration was performed. Then, validation tests for capture ELISAs were performed to assess lower limit of detection (LLOD), matrix effect, parallelism, and inter-assay variation.
To optimize antibody concentrations, checkerboard titration was performed. Then, validation tests for capture ELISAs were performed to assess lower limit of detection (LLOD), matrix effect, parallelism, and inter-assay variation.
| Date made available | 9 Dec 2022 |
|---|---|
| Publisher | Wageningen University & Research |
| Temporal coverage | 2018 - 2019 |
Keywords
- chicken
- poultry
- capture ELISA
- antibodies
- cytokines
- interleukin
- interferon
Research output
- 1 Article
-
Optimization of Capture ELISAs for Chicken Cytokines Using Commercially Available Antibodies
Krzysica, P., Verhoog, L., de Vries, S., Smits, C., Savelkoul, H. F. J. & Tijhaar, E., 4 Nov 2022, In: Animals. 12, 21, 3040.Research output: Contribution to journal › Article › Academic › peer-review
Open Access6 Citations (Scopus)