Data from: Soil microeukaryotic communities and phosphorus-cycling microorganisms respond to chloropicrin fumigation and azoxystrobin application

Fumigants and fungicides are effective at controlling soil-borne pathogens
but might also adversely affect soil beneficial microbes, such as soil
phosphorus (P) solubilizing microbes, further altering nutrient cycling
processes. Therefore, this study investigated the effects of the fumigant
chloropicrin (CP) and the fungicide azoxystrobin (AZO) on soil
microeukaryotes and P-cycling related soil bacteria through a greenhouse
experiment. Soil microeukaryotic communities and bacterial communities
containing two phosphomonoesterase encoding genes (phoC and phoD) were
analysed using high-throughput sequencing methods. Results showed that,
when applied at the field recommended application dosage, the fungicide
AZO had no significant influence on the community structure of soil
microeukaryotes and phoD-containing bacteria. However, in CP-fumigated
soils, the soil microeukaryotic community composition changed from
fungi-dominated to protist-dominated. CP fumigation significantly
decreased the total phoC/phoD gene copy number but increased the relative
abundance of some phoC/phoD-containing bacteria (such
as Sinorhizobium and Streptomyces), which are significantly positively
correlated to available P compositions in soil. The structural equation
model (SEM) confirmed that CP fumigation could affect soil available P
content directly by altering phoC-/phoD-containing bacteria, or indirectly
by affecting phoC/phoD gene abundance and acid/alkaline phosphatases
activity in soil. The inconsistent changes in phoC/phoD-containing
bacteria, phoC/phoD gene number, and the phosphomonoesterase activities
indicated that enzyme secretion may not be the only way for P solubilizing
soil microorganisms to regulate P availability after soil fumigation. The
outcome of this study can provide theoretical support for the design of
soil beneficial microorganism recovery strategies and the regulation of
phosphate fertilizer after soil fumigation.