Data from: Development of a Nasonia vitripennis outbred laboratory population for genetic analysis



The parasitoid wasp genus Nasonia has rapidly become a genetic model system for developmental and evolutionary biology. The release of its genome sequence led to the development of high-resolution genomic tools, for both interspecific and intraspecific research, which has resulted in great advances in understanding Nasonia biology. To further advance the utility of Nasonia vitripennis as a genetic model system and to be able to fully exploit the advantages of its fully sequenced and annotated genome, we developed a genetically variable and well-characterized experimental population. In this study, we describe the establishment of the genetically diverse HVRx laboratory population from strains collected from the field in the Netherlands. We established a maintenance method that retains genetic variation over generations of culturing in the laboratory. As a characterization of its genetic composition, we provide data on the standing genetic variation and estimate the effective population size (Ne) by microsatellite analysis. A genome-wide description of polymorphism is provided through pooled resequencing, which yielded 417 331 high-quality SNPs spanning all five Nasonia chromosomes. The HVRx population and its characterization are freely available as a community resource for investigators seeking to elucidate the genetic basis of complex trait variation using the Nasonia model system.
Date made available12 Nov 2013
PublisherUniversity of Groningen

Cite this

van de Zande, L. (Creator), Ferber, S. (Creator), de Haan, A. (Creator), Beukeboom, L. W. (Creator), van Heerwaarden, J. (Creator), Pannebakker, B. A. (Creator) (12 Nov 2013). Data from: Development of a Nasonia vitripennis outbred laboratory population for genetic analysis. University of Groningen. 10.5061/dryad.f2330