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assessment of Cas9-driven genome editing in presence of DNA oligos in CML cells

  • Thomas Swartjes (Creator)
  • Peng Shang (Creator)
  • Dennis van den Berg (Creator)
  • Tim Künne (Creator)
  • Niels Geijsen (Creator)
  • Stan J.J. Brouns (Delft University of Technology) (Creator)
  • John van der Oost (Creator)
  • Raymond Staals (Creator)
  • Richard Notebaart (Creator)

Dataset

Description

We assessed indels resulting from Cas9-induced genome editing in CML cells in the presence of guide-complementary DNA oligonucleotides intended to inhibit Cas9 activity. We amplified pre-determined loci that were target with the supplied guides or were expected to be off-target sites for these guides. The on-target loci are EMX1 and FANCF.
Date made available13 Jan 2022
PublisherWageningen University & Research

Accession numbers

  • PRJNA796802

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