Aspergillus fumigatus - Aspergillus fumigatus differential expression upon itraconazole addition

  • Margriet W.J. Hokken (Creator)
  • Jan Zoll (Creator)
  • Jordy P.M. Coolen (Creator)
  • Bas Zwaan (Creator)
  • Paul E. Verweij (Creator)
  • Willem J.G. Melchers (Creator)



The filamentous fungus Aspergillus fumigatus is an opportunistic pathogen which causes life-threatening diseases in immunocompromised patients. Resistance of A. fumigatus against azole class compounds continues to pose a threat to human health worldwide. How this fungus maintains fitness before any resistance causing mutations arise is not well understood. In this study, we use RNA-seq to demonstrate how A. fumigatus exerts its phenotypic plasticity when exposed to itraconazole, and which important cellular processes contribute to its adaptation to a new, stressful environment containing azole compounds. We conducted an in vitro assay in which we exposed 24h old mycelia of clinical A. fumigatus isolates grown at 37°C, to sublethal concentrations of itraconazole which might permit gradual adaptation of the fungus to the new, stressful environment. Mycelia were then harvested after 30, 60, 120 or 240 minutes respectively, and relative expression was compared to the mycelia harvested directly after 24h without the addition of itraconazole
Date made available24 Jul 2018

Research Output

Phenotypic plasticity and the evolution of azole resistance in Aspergillus fumigatus; An expression profile of clinical isolates upon exposure to itraconazole

Hokken, M. W. J., Zoll, J., Coolen, J. P. M., Zwaan, B. J., Verweij, P. E. & Melchers, W. J. G., 9 Jan 2019, In : BMC Genomics. 20, 1, 28.

Research output: Contribution to journalArticleAcademicpeer-review

Open Access
  • 5 Citations (Scopus)

    Cite this

    Hokken, M. W. J. (Creator), Zoll, J. (Creator), Coolen, J. P. M. (Creator), Zwaan, B. (Creator), Verweij, P. E. (Creator), Melchers, W. J. G. (Creator) (24 Jul 2018). Aspergillus fumigatus - Aspergillus fumigatus differential expression upon itraconazole addition