Activated Carbon mixed with marine sediment suits as bioanode material for Spartina anglica Sediment/Plant Microbial Fuel Cell: plant growth, electricity generation and spatial less microbial community diversity

Dataset

Description

This study is part of paper: "Activated Carbon mixed with marine sediment suits as bioanode material for Spartina anglica Sediment/Plant Microbial Fuel Cell: plant growth, electricity generation and spatial microbial community diversity" In this study, eight lab-wetlands systems in the shape of flat-plate Plant-MFC were constructed. Spartina anglica model plant was planted in the anode chamber of the Plant-MFC reactors. Here, four wetlands compositions with activated carbon and/or marine sediment functioning as anodes were investigated for their suitability as a bioanode in a Plant-MFC system.Four different anode compositions were used to fill the anode compartments (650ml). Plant-MFC 1 and Plant-MFC 2, this duplicate was named as AC100, were filled with 100% activated carbon (AC); Plant-MFC 3 and Plant-MFC 4, this duplicate was named as MS100, were only filled with marine sediment; Plant-MFC 5 and Plant-MFC 6, this duplicate was named as AC67, were filled with a mixture of 67 % AC and 33% marine sediment; and Plant-MFC 7 and Plant-MFC 8, this duplicate was named as AC33, were filled with a mixture of 33% AC and 67% marine sediment. The utilized AC is granular activated carbon PK 1-3(Cabot Norit Netherlands BV, with apparent density of 290 g/L)At the end of the experiment (day 190), about 3 ml biomass samples from anode components (mixture of marine sediment, AC and plant roots) were taken for DNA analysis. Samples were taken from the MS100 (Plant-MFC 3 & Plant-MFC 4) and the AC33 (Plant-MFC 7 & Plant-MFC 8). For every reactor, five biomass samples were collected. Biomass samples were taken from five different locations in the anode. These five sample locations were clustered in two zones: upper zone (until 5cm below the anode surface) and lower zone (from 5 until 20 cm below the anode surface). The upper zone(UZ) sample points were (A) UZ-AN (anode) and (C) UZ-CC (current collector). The lower zone sample points were (B) LZ-RO (roots), (D) LZ-AN (anode), and (E) LZ-CC (current collector). In the MS100 plant-MFC, the anode biomass samples (UZ-AN and LZ-AN) contained marine sediment; the current collector biomass samples (UZ-CC and LZ-CC) contained marine sediment that were attached on the current collector. While, in the AC33 plant-MFC, the anode biomass samples (UZ-AN and LZ-AN) contained AC and marine sediment; the current collector biomass samples (UZ-CC and LZ-CC) contained AC and marine sediment that were attached on the current collector. In total 20 samples were collected. The samples were stored immediately in -80 Centigrade freezer after collection before the DNA sequencing was performed.Further detail information can be found in the above mentioned paper.
Date made available8 Oct 2019
PublisherWageningen University

Cite this

Sudirjo, E. (Creator), Buisman, C. J. N. (Creator), Strik, D. P. B. T. B. (Creator)(8 Oct 2019). Activated Carbon mixed with marine sediment suits as bioanode material for Spartina anglica Sediment/Plant Microbial Fuel Cell: plant growth, electricity generation and spatial less microbial community diversity. Wageningen University.