A Robust Auxin Response Network Controls Embryo and Suspensor Development through a bHLH Transcriptional Module

Dataset

Description

Land plants can reproduce sexually by developing an embryo from a fertilized egg cell. However, embryos can also be formed from other cell types in many plant species. A key question is thus how embryo identity in plants is controlled, and how this process is modified during non-zygotic embryogenesis. The Arabidopsis zygote divides to produce an embryonic lineage and an extra-embryonic suspensor. Yet, normally quiescent suspensor cells can develop a second embryo when the initial embryo is damaged, or when response to the signaling molecule auxin is locally blocked. Here we have used auxin-dependent suspensor embryogenesis as a model to determine transcriptome changes during embryonic reprogramming. We find that reprogramming is complex and accompanied by large transcriptomic changes prior to anatomic changes. This analysis revealed a strong enrichment for genes encoding components of auxin homeostasis and response among misregulated genes. Strikingly, deregulation among multiple auxin-related gene families converged upon re-establishment of cellular auxin levels or response. This suggests a remarkable degree of feedback regulation to create resilience in auxin response during embryo development. Starting from the transcriptome of auxin-deregulated embryos, we identify an auxin-dependent bHLH transcription factor network that mediates the activity of this hormone in suppressing embryo development from the suspensor.
Date made available3 Jan 2019
PublisherWageningen University

Research Output

A Robust Auxin Response Network Controls Embryo and Suspensor Development through a bHLH Transcriptional Module

Radoeva, T., Lokerse, A. S., Llavata-Peris, C. I., Wendrich, J., Xiang, D., Liao, C-Y., Vlaar, L., Boekschoten, M., Hooiveld, G., Datla, R. & Weijers, D., Jan 2019, In : The Plant Cell. 31, p. 52-67

Research output: Contribution to journalArticleAcademicpeer-review

Open Access
  • 8 Citations (Scopus)

    Cite this

    Radoeva, T. M. (Creator), Boekschoten, M. V. (Creator), Hooiveld, G. J. E. J. (Creator), Weijers, D. (Creator) (3 Jan 2019). A Robust Auxin Response Network Controls Embryo and Suspensor Development through a bHLH Transcriptional Module. Wageningen University.