Research Output per year
A common bacterial strategy for monitoring environmental challenges is to use two-component systems, which consist of a sensor histidine kinase (HK) and a response regulator (RR). In the food-borne pathogen Bacillus cereus, the alternative sigma factor σB is activated by the RR RsbY. Here we present strong indications that the PP2C-type phosphatase RsbY receives its input from the multi-sensor hybrid kinase BC1008 (renamed RsbK). Genome analyses revealed that, across bacilli, rsbY and rsbK are located in a conserved gene cluster. A B. cereus rsbK deletion strain was shown to be incapable of inducing σB upon stress conditions and was impaired in its heat adaptive response. Comparison of the wild-type and rsbK mutant transcriptomes upon heat shock revealed that RsbK was primarily involved in the activation of the σB-mediated stress response. Truncation of the RsbK RR receiver domain demonstrated the importance of
|Date made available||17 Dec 2009|
A novel hybrid kinase is essential for regulating the sigmaB-mediated stress response of Bacillus cereusde Been, M. W. H. J., Tempelaars, M. H., van Schaik, W., Moezelaar, R., Siezen, R. J. & Abee, T., 2010, In : Environmental Microbiology. 12, 3, p. 730-745
Research output: Contribution to journal › Article › Academic › peer-review
de Been, M. W. H. J. (Creator), Tempelaars, M. (Creator), van Schaik, W. (Creator), Moezelaar, R. (Creator), Siezen, R. J. (Creator), Abee, T. (Creator) (17 Dec 2009). A novel hybrid kinase is essential for regulating the σB-mediated stress response of Bacillus cereus. Wageningen UR.