β2→1-fructans modulate the immune system in vivo in a microbiota-dependent and -independent fashion

  • Floris Fransen (Creator)
  • Neha M. Sahasrabudhe (Creator)
  • Marlies Elderman (Creator)
  • Margaret Bosveld (Creator)
  • Sahar El Aidy (Creator)
  • F. Hugenholtz (Creator)
  • Theo Borghuis (Creator)
  • Ben Kousemaker (Creator)
  • Simon Winkel (Creator)
  • Christa van der Gaast-de Jongh (Creator)
  • Marien I. De Jonge (Creator)
  • Mark Boekschoten (Creator)
  • Hauke Smidt (Creator)
  • Paul de Vos (Creator)

Dataset

Description

It has been shown in vitro that only specific dietary-fibers contribute to immunity but studies in vivo are not conclusive. Here we investigated degree of polymerization (DP) dependent effects of β2→1-fructans on immunity via microbiota-dependent and -independent effects. To this end, conventional or germ-free mice received short- or long-chain β2→1-fructan for 5 days. Immune cell populations in the spleen, mesenteric lymph nodes (MLN), and Peyer's patches (PPs) were analyzed with flow cytometry, genome-wide gene expression in the ileum was measured with microarray, and gut microbiota composition was analyzed with 16S rRNA sequencing of fecal samples. We found that β2→1-fructans modulated immunity by both microbiota and microbiota-independent effects. Moreover, effects were dependent on the chain-length of the β2→1-fructans type polymer. Both short- and long-chain β2→1-fructans enhanced T-helper 1 cells in Peyer's patches, whereas only short-chain β2→1-fructans increased regulatory T cells and CD11b-CD103- DCs in the MLN. A common feature after short- and long-chain β2→1-fructan treatment was enhanced Fut2 expression and other IL-22-dependent genes in the ileum of conventional mice. These effects were not associated with shifts in gut microbiota composition, or altered production of short-chain fatty acids. Both short- and long-chain β2→1-fructans also induced immune effects in germ-free animals, demonstrating direct effect independent from the gut microbiota. Also, these effects were dependent on the chain-length of the β2→1-fructans. Short-chain β2→1-fructan induced lower CD80 expression by CD11b-CD103- DCs in PPs, whereas long-chain β2→1-fructan specifically modulated B cell responses in germ-free mice. In conclusion, support of immunity is determined by the chemical structure of β2→1-fructans and is partially microbiota-independent.
Date made available22 Mar 2018
PublisherWageningen University

Research Output

β2→1-fructans modulate the immune system in vivo in a microbiota-dependent and -independent fashion

Fransen, F., Sahasrabudhe, N. M., Elderman, M., Bosveld, M., El Aidy, S., Hugenholtz, F., Borghuis, T., Kousemaker, B., Winkel, S., van der Gaast-de Jongh, C., de Jonge, M. I., Boekschoten, M. V., Smidt, H., Schols, H. A. & de Vos, P., 2017, In : Frontiers in Immunology. 8, 154.

Research output: Contribution to journalArticleAcademicpeer-review

Open Access
  • 22 Citations (Scopus)

    Cite this

    Fransen, F. (Creator), Sahasrabudhe, N. M. (Creator), Elderman, M. (Creator), Bosveld, M. (Creator), El Aidy, S. (Creator), Hugenholtz, F. (Creator), Borghuis, T. (Creator), Kousemaker, B. (Creator), Winkel, S. (Creator), van der Gaast-de Jongh, C. (Creator), de Jonge, M. I. (Creator), Boekschoten, M. V. (Creator), Smidt, H. (Creator), de Vos, P. (Creator) (22 Mar 2018). β2→1-fructans modulate the immune system in vivo in a microbiota-dependent and -independent fashion. Wageningen University.