DescriptionCyst nematodes are considered a dominant threat to yield for a wide range of major food crops. Current control strategies of cyst nematodes mainly dependent on crop rotation and the use of resistant crop cultivars. Various crops exhibit single dominant resistance (R) genes able to activate effective host-specific resistance to certain cyst nematode species and/or populations. An example is the potato R gene Gpa2, which confers resistance against the potato cyst nematode Globodera pallida. Gpa2 encodes an intracellular nucleotide binding-leucine-rich repeat (NB-LRR) immune receptor, which is able to specifically recognize the effector GpRBP-1. However, knowledge about the cellular and molecular mechanisms underlying effector detection and the activation of plant immune responses is still largely unknown. Here, we show that effector detection by Gpa2 occurs in the cytoplasm and that both the nuclear and cytoplasmic pools of Gpa2 contribute to effector-triggered immunity in cell death assays and nematode infection tests on transgenic potato roots. Moreover, a comparative transcriptome study was conducted to uncover differential gene expression patterns upon infection of Gpa2 resistant potato roots using virulent and avirulent nematode populations. This revealed specific DEGs involved in the activation of plant defence pathways linked to calcium binding and metabolic activity, systemic acquired resistance, salicylic acid biosynthesis and immune responses.
|Period||24 Aug 2023|
|Event title||12th International Congress of Plant Pathology (2023): One Health for all Plants, Crops and Trees|